How to Prepare a Cheek Cell Slide
Preparation of a cheek cell slide is a fundamental technique in cytology and is commonly used in educational settings to introduce students to the study of cells. This process involves collecting cells from the inside of the cheek, preparing them for microscopic examination, and then mounting them on a slide. Below is a step-by-step guide on how to prepare a cheek cell slide.
Materials Needed:
Before beginning, gather all the necessary materials. You will need:
– A sterile wooden tongue depressor or a cotton swab
– Saline solution (0.9% sodium chloride)
– A glass microscope slide
– A cover slip
– A dropper
– Ethanol or 70% isopropyl alcohol
– A stain (e.g., methylene blue or Giemsa)
– A light source or microscope
Step 1: Collecting the Cells
1. Clean the inside of your cheek with a sterile wooden tongue depressor or a cotton swab.
2. Gently rub the cheek in a circular motion to collect cells.
3. Transfer the cells to a clean glass microscope slide.
Step 2: Adding Saline Solution
1. Add a few drops of saline solution to the slide containing the cheek cells.
2. Gently spread the cells across the slide using the edge of the tongue depressor or a cotton swab.
Step 3: Staining the Cells
1. Allow the cells to air dry completely.
2. Once dry, add a few drops of stain to the slide.
3. Let the stain sit on the cells for a few minutes to color them.
Step 4: Mounting the Slide
1. Once the stain has been absorbed, gently blot the excess with a paper towel.
2. Place a cover slip over the stained cells, ensuring there are no air bubbles.
3. Press down gently to secure the cover slip in place.
Step 5: Storing the Slide
1. Label the slide with your name and the date.
2. Store the slide in a safe place, away from direct sunlight and extreme temperatures.
Conclusion
Preparing a cheek cell slide is a simple yet effective way to observe cells under a microscope. By following these steps, you can create a slide that is ready for examination and contribute to your understanding of cellular structure and function. Remember to practice proper hygiene and safety measures throughout the process to ensure accurate results.
